Journal
ACS CATALYSIS
Volume 9, Issue 12, Pages 11658-+Publisher
AMER CHEMICAL SOC
DOI: 10.1021/acscatal.9b04819
Keywords
galactose oxidase; continuous flow; carbohydrate dialdehydes; glucosides; polymer
Categories
Funding
- RCUK [BB/L013762/1, BB/M027791/1, BB/M02903411, BB/M028836/1]
- ERC [788231-ProgrES-ERC-2017-ADG]
- UK Catalysis Hub
- Engineering and Physical Sciences Research Council (EPSRC) (Future BRH grant)
- IBioIC
- Prozomix Ltd.
- Bioshape Ltd.
- BBSRC [BB/M028836/1, BB/L013762/1, BB/M027791/1, 1960946] Funding Source: UKRI
- EPSRC [EP/M013219/1, EP/S01778X/1] Funding Source: UKRI
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Galactose Oxidase (GOase) has been used for the scalable and selective C-6' oxidation of lactose, a waste material from the dairy industry. Generation of the 6'-oxo lactose was achieved with full conversion in batch mode at milligram scale, but further scale-up to gram quantities proved to be challenging because of requirements for high enzyme concentrations and limitation in oxygen cosubstrate availability. To overcome these issues, a continuous-flow system was developed for the bio-oxidation of lactose yielding multigram quantities of product. Using the variant GOase F-2, terminal selective oxidations were also observed on a range of oligoglucosides such as maltose. The carbohydrate dialdehydes that were obtained by this highly selective oxidation were chemically further functionalized, establishing the biooxidation as a route to valorize cheap carbohydrates, including waste materials, for building blocks of polymers.
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