Journal
NATURE COMMUNICATIONS
Volume 10, Issue -, Pages -Publisher
NATURE PUBLISHING GROUP
DOI: 10.1038/s41467-019-12480-3
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Funding
- Max Planck Society
- Ecole Polytechnique Federale de Lausanne
- Swiss Commission for Technology and Innovation (CTI)
- NCCR Chemical Biology
- European Molecular Biology Laboratory
- EMBL International PhD Program
- European Research Council [ERC CoG-724489]
- National Institutes of Health Common Fund 4D Nucleome Program [U01 EB021223/U01, DA047728]
- Advanced Light Microscopy Facility (ALMF) at the European Molecular Biology Laboratory (EMBL)
- Leica Microsystems for support
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Photoactivatable fluorophores are important for single-particle tracking and super-resolution microscopy. Here we present a photoactivatable fluorophore that forms a bright silicon rhodamine derivative through a light-dependent protonation. In contrast to other photoactivatable fluorophores, no caging groups are required, nor are there any undesired side-products released. Using this photoactivatable fluorophore, we create probes for HaloTag and actin for live-cell single-molecule localization microscopy and single-particle tracking experiments. The unusual mechanism of photoactivation and the fluorophore's outstanding spectroscopic properties make it a powerful tool for live-cell super-resolution microscopy.
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