Journal
NATURE COMMUNICATIONS
Volume 10, Issue -, Pages -Publisher
NATURE PUBLISHING GROUP
DOI: 10.1038/s41467-019-12027-6
Keywords
-
Categories
Funding
- DTRA [HDTRA1-13-0037]
- NSF [1435957, 1807604, 1805274]
- National Institutes of Health [R21EB024102]
- Directorate For Engineering
- Div Of Chem, Bioeng, Env, & Transp Sys [1805274, 1435957] Funding Source: National Science Foundation
- Directorate For Engineering
- Div Of Electrical, Commun & Cyber Sys [1807604] Funding Source: National Science Foundation
Ask authors/readers for more resources
Synthetic biology and metabolic engineering have expanded the possibilities for engineered cell-based systems. The addition of non-native biosynthetic and regulatory components can, however, overburden the reprogrammed cells. In order to avoid metabolic overload, an emerging area of focus is on engineering consortia, wherein cell subpopulations work together to carry out a desired function. This strategy requires regulation of the cell populations. Here, we design a synthetic co-culture controller consisting of cell-based signal translator and growth-controller modules that, when implemented, provide for autonomous regulation of the consortia composition. The system co-opts the orthogonal autoinducer Al-1 and Al-2 cell-cell signaling mechanisms of bacterial quorum sensing (QS) to enable crosstalk between strains and a QS signal-controlled growth rate controller to modulate relative population densities. We further develop a simple mathematical model that enables cell and system design for autonomous closed-loop control of population trajectories.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available