Journal
NATURE COMMUNICATIONS
Volume 10, Issue -, Pages -Publisher
NATURE PUBLISHING GROUP
DOI: 10.1038/s41467-019-12304-4
Keywords
-
Categories
Funding
- NIH [R01-GM131099, R01-GM124472]
- NSF CAREER Award [1350829]
- National Science Foundation Graduate Research Fellowship Program [DGE-1444932, NCI-1F99CA234959-01]
- Emory Comprehensive Glycomics Core
- National Center for Advancing Translational Sciences of the National Institutes of Health [UL1TR000454]
- National Center for Advancing Translational Sciences of the National Institute of Health [UL1TR002378]
- ANR agency
- LLNC
- FRM as Equipe labellisee
Ask authors/readers for more resources
Podosomes are ubiquitous cellular structures important to diverse processes including cell invasion, migration, bone resorption, and immune surveillance. Structurally, podosomes consist of a protrusive actin core surrounded by adhesion proteins. Although podosome protrusion forces have been quantified, the magnitude, spatial distribution, and orientation of the opposing tensile forces remain poorly characterized. Here we use DNA nanotechnology to create probes that measure and manipulate podosome tensile forces with molecular piconewton (pN) resolution. Specifically, Molecular Tension-Fluorescence Lifetime Imaging Microscopy (MT-FLIM) produces maps of the cellular adhesive landscape, revealing ring-like tensile forces surrounding podosome cores. Photocleavable adhesion ligands, breakable DNA force probes, and pharmacological inhibition demonstrate local mechanical coupling between integrin tension and actin protrusion. Thus, podosomes use pN integrin forces to sense and respond to substrate mechanics. This work deepens our understanding of podosome mechanotransduction and contributes tools that are widely applicable for studying receptor mechanics at dynamic interfaces.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available