Journal
BIOTECHNOLOGY LETTERS
Volume 39, Issue 1, Pages 97-104Publisher
SPRINGER
DOI: 10.1007/s10529-016-2231-z
Keywords
Acetone/butanol/ethanol fermentation; Acid crash; Calcium carbonate; Clostridium acetobutylicum; Formic acid; Lignocellulosic hydrolysate; PH adjustment methods
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Funding
- National Natural Science Foundation of China [51378486, 51508547, 21606229, 31600475]
- Science and Technology Project of Huaian [HAS2015035]
- Natural Science Foundation of Guangdong Province, China [2016A030310124]
- Science and Technology Planning Project of Guangdong Province, China [2016A010104009, 2016A010105016]
- Youth Innovation Promotion Association CAS [2015290]
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To investigate the inhibiting effect of formic acid on acetone/butanol/ethanol (ABE) fermentation and explain the mechanism of the alleviation in the inhibiting effect under CaCO3 supplementation condition. From the medium containing 50 g sugars l(-1) and 0.5 g formic acid l(-1), only 0.75 g ABE l(-1) was produced when pH was adjusted by KOH and fermentation ended prematurely before the transformation from acidogenesis to solventogenesis. In contrast, 11.4 g ABE l(-1) was produced when pH was adjusted by 4 g CaCO3 l(-1). The beneficial effect can be ascribed to the buffering capacity of CaCO3. Comparative analysis results showed that the undissociated formic acid concentration and acid production coupled with ATP and NADH was affected by the pH buffering capacity of CaCO3. Four millimole undissociated formic acid was the threshold at which the transformation to solventogenesis occurred. The inhibiting effect of formic acid on ABE fermentation can be alleviated by CaCO3 supplementation due to its buffering capacity.
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