Journal
BIOTECHNOLOGY LETTERS
Volume 38, Issue 5, Pages 817-823Publisher
SPRINGER
DOI: 10.1007/s10529-016-2041-3
Keywords
Biotransformation; delta-Decalactone; 13-Hydroxy-9(Z)-octadecenoic acid; Linoleate 13-hydratase; Linoleic acid; Yarrowia lipolytica
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Funding
- Korea Healthcare Technology R and D Project, Ministry for Health and Welfare, Republic of Korea [2012-009]
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Objective To produce delta-decalactone from linoleic acid by one-pot reaction using linoleate 13-hydratase with supplementation with whole Yarrowia lipolytica cells. Results Whole Y. lipolytica cells at 25 g l(-1) produced1.9 g l(-1) delta-decalactone from 7.5 g 13-hydroxy-9(Z)-octadecenoic acid l(-1) at pH 7.5 and 30 A degrees C for 21 h. Linoleate 13-hydratase from Lactobacillus acidophilus at 3.5 g l(-1) with supplementation with 25 g Y. lipolytica cells l(-1) in one pot at 3 h produced 1.9 g l(-1) delta-decalactone from 10 g linoleic acid l(-1) via 13-hydroxy-9(Z)-octadecenoic acid intermediate at pH 7.5 and 30A degrees C after 18 h, with a molar conversion yield of 31 % and productivity of 106 mg l(-1) h(-1). Conclusion To the best of our knowledge, this is the first production of delta-decalactone using unsaturated fatty acid.
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