Journal
BIOANALYSIS
Volume 11, Issue 19, Pages 1737-1754Publisher
FUTURE SCI LTD
DOI: 10.4155/bio-2019-0190
Keywords
blood; hematocrit; LC-MS; microsampling; Mitra R; microsamplers; pediatric; pharmacokinetics; volumetric absorptive sampling (VAMS); wet vs dry
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Aim: Collection and quantitative analysis in dry blood using volumetric absorptive microsampling (VAMST) potentially offers significant advantages over conventional wet whole blood analysis. This manuscript explores their use for pediatric sampling and explores additional considerations for the validation of the bioanalytical method. Results: HPLC-MS/MS methods for the determination of midazolam and its major metabolite 1-OH midazolam in both whole wet blood, and dry blood collected on VAMS were developed, validated, and used to support an observational clinical study to compare pharmacokinetic parameters in pediatric patients. Conclusion: Validation data met internationally accepted guideline criteria. A strong correlation was observed in calculated concentrations between wet and dry test samples, indicating that VAMS is a suitable technique for use in pediatric clinical studies.
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