Journal
BIOANALYSIS
Volume 11, Issue 19, Pages 1799-1812Publisher
FUTURE SCI LTD
DOI: 10.4155/bio-2019-0145
Keywords
bacterial cellulose; biomarker; cibacron blue; combinatorial peptide ligand library (CPLL); cryogels; depletion; molecular imprinted polymers; plasma; proteomics; serum
Funding
- Arus Perdana Research Grant - Universiti Kebangsaan Malaysia [AP-2017-007/3]
- Long Term Research Grant Scheme - Ministry of Education Malaysia [LRGS/2014/UKM-UKM/K/01]
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Plasma and serum are widely used for proteomics-based biomarker discovery. However, analysis of these biofluids is highly challenging due to the complexity and wide dynamic range of their proteomes. Notably, highly abundant proteins tend to obscure the detection of potential biomarkers that are usually of lower concentrations. Among the strategies to resolve this problem are: depletion of high-abundance proteins, enrichment of low abundant proteins of interest and prefractionation. In this review, we focus on current and emerging depletion techniques used to enhance the detection and identification of the less abundant proteins in plasma and serum. We discuss the applications and contributions of these methods to proteomics analysis of plasma and serum alongside their limitations and future perspectives.
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