Journal
WORLD JOURNAL OF MICROBIOLOGY & BIOTECHNOLOGY
Volume 35, Issue 11, Pages -Publisher
SPRINGER
DOI: 10.1007/s11274-019-2748-0
Keywords
Odd-chain fatty acid; 1-Propanol; Rhodococcus opacus; Microbial oil; methylmalonyl-CoA pathway
Categories
Funding
- National Natural Science Foundation of China [21676104, 21878105]
- National Key Research and Development Program of China [2018YFC1602100, 2018YFC1603400]
- Science and Technology Program of Guangzhou [201904010360]
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Odd-chain fatty acids (OCFAs) have been reported to possess pharmacological activity and have been used in the manufacture of agricultural and industrial chemicals. We here provided a new method to increase the OCFAs content in oil produced by Rhodococcus opacus PD630 through addition of 1-propanol to the fermentation media. The OCFAs in oil of R. opacus PD630 are primarily pentadecanoic acid (C15:0), heptadecanoic acid (C17:0) and heptadecenoic acid (C17:1). After adding 0.5-1.5% (v/v) 1-propanol, the production of oil increased from 1.27 g/L to 1.31-1.61 g/L, and the OCFAs content in oil increased by 46.7-55.1%. Metabolic intermediates determination and transcriptome analysis revealed that R. opacus assimilated 1-propanol through methylmalonyl-CoA pathway. When the nitrogen source was limited, propionyl-CoA was converted to propionyl-acyl carrier protein (ACP) which could be used as primer during the elongation of fatty acid synthesis. Then OCFAs were produced when odd number of propionyl-ACP was incorporated in the cycles of fatty acid synthesis. [GRAPHICS] .
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