Journal
VIRUS RESEARCH
Volume 273, Issue -, Pages -Publisher
ELSEVIER
DOI: 10.1016/j.virusres.2019.197767
Keywords
Human coronavirus NL63; Sambucus FormosanaNakai; Caffeic acid; Antiviral; Virus yield; Attachment inhibition
Categories
Funding
- China Medical University under the Featured Areas Research Center Program within the framework of the Higher Education Sprout Project, Ministry of Education, Taiwan [CHM106-6-2, CMRC-CHM-2]
- Ministry of Science and Technology, Taiwan [MOST107-2923-B-039-001-MY3, MOST105-2320-B-039-053-MY3]
- China Medical University [CMU106-BC-1, CMU106-ASIA-06, CMU107-ASIA-12, CMU107-S-14]
Ask authors/readers for more resources
Human coronavirus NL63 (HCoV-NL63), one of the main circulating HCoVs worldwide, causes respiratory tract illnesses like runny nose, cough, bronchiolitis and pneumonia. Recently, a severe respiratory illness outbreak of HCoV-NL63 has been reported in a long-term care facility. Sambucus FormosanaNakai, a species of elderberry, is a traditional medicinal herb with anti-inflammatory and antiviral potential. The study investigated the antiviral activity of Sambucus FormosanaNakai stem ethanol extract and some phenolic acid constituents against HCoV-NL63. The extract was less cytotoxic and concentration-dependently increased anti-HCoV-NL63 activities, including cytopathicity, sub-G1 fraction, virus yield (IC50 = 1.17 mu g/ml), plaque formation (IC50 = 4.67 mu g/ml) and virus attachment (IC50 = 15.75 mu g/ml). Among the phenolic acid constituents in Sambucus FormosanaNakai extract, caffeic acid, chlorogenic acid and gallic acid sustained the anti-HCoV-NL63 activity that was ranked in the following order of virus yield reduction: caffeic acid (IC50 = 3.54 mu M) > chlorogenic acid (IC50 = 43.45 mu M) > coumaric acid (IC50 = 71.48 mu M). Caffeic acid significantly inhibited the replication of HCoV-NL63 in a cell-type independent manner, and specifically blocked virus attachment (IC50 = 8.1 mu M). Therefore, the results revealed that Sambucus Formosana Nakai stem ethanol extract displayed the strong anti-HCoV-NL63 potential; caffeic acid could be the vital component with anti-HCoV-NL63 activity. The finding could be helpful for developing antivirals against HCoV-NL63.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available