Journal
BIOTECHNOLOGY AND BIOPROCESS ENGINEERING
Volume 21, Issue 6, Pages 777-786Publisher
KOREAN SOC BIOTECHNOLOGY & BIOENGINEERING
DOI: 10.1007/s12257-016-0202-9
Keywords
Bacillus natto; menaquinone-7; extraction; optimization; sporulation ratio
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Funding
- National Science Foundation for Distinguished Young Scholars of China [21225626]
- National Natural Science Foundation of China [21306085, 21476111]
- National High Technology Research and Development Program of China [2014AA021701]
- Specialized Research Fund for Doctoral Program of Higher Education [20133221120008]
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Vitamin K-2 (menaquinone or MK) plays an important role in blood clotting, cardiovascular disease, and anti-osteoporosis. A novel bacterial strain was isolated and identified as Bacillus natto based on 16SrDNA sequencing and LC-MS analysis. The objective of this study was to improve the extraction efficiency and productivity of MK-7 from B. natto. Acid-heating method efficiently disrupted B. natto cells for MK-7 extraction. Bacillus natto had a wide range of pH (5.0 similar to 9.0) for optimal growth. Its MK-7 yield was increased when rotation speed was increased to 200 rpm. The highest MK-7 yield was obtained when glycerol and soy peptone were used in the growth media. Batch fermentation was subsequently tested in 5 L bioreactor, which gave a high productivity of MK-7 (at 0.60 mg/L/h). A positive correlation between MK-7 yield and sporulation ratio was also found. This study provides valuable information on the extraction and production of menaquinone-7 from B. natto under submerged fermentation condition.
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