Journal
BIOTECHNOLOGY AND BIOPROCESS ENGINEERING
Volume 21, Issue 3, Pages 402-407Publisher
KOREAN SOC BIOTECHNOLOGY & BIOENGINEERING
DOI: 10.1007/s12257-016-0197-2
Keywords
acetate; ethanol; metabolic engineering; Ralstonia eutropha
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Funding
- New & Renewable Energy Program of the Korea Institute of Energy Technology Evaluation and Planning [20133030000300]
- National Research Foundation of Korea - Korean Government [2012M1A2A2026560]
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This study was performed to produce ethanol from acetate using a genetically engineered Ralstonia eutropha. In order to genetically modify R. eutropha H16, phaCAB operon encoding metabolic pathway genes from acetyl-CoA to polyhydroxybutyrate (PHB) was deleted and adhE encoding an alcohol dehydrogenase from Escherichia coli was overexpressed for conversion of acetyl-CoA to ethanol. The resulting strain produced ethanol up to 170 mg/L when cultivated in minimal media supplemented with 5 g/L of acetate as a sole carbon source. Growth and ethanol production were optimized by adjusting nitrogen source (NH4Cl) content and repetitive feeding of acetate into the bacterial culture, by which the ethanol production was reached to approximately 350 mg/L for 84 h.
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