Journal
BIOTECHNIQUES
Volume 61, Issue 1, Pages 20-25Publisher
BIOTECHNIQUES OFFICE
DOI: 10.2144/000114432
Keywords
LAMP; isothermal amplification; SYTO-9; SYTO-13; SYTO-82; SYBR Green I; SYBR Gold; EvaGreen; Bst DNA polymerase
Funding
- State Federal Budget Program [62.2.3]
- RAS program [(I.30) FIMT 153]
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Real-time or quantitative loop-mediated isothermal amplification (qLAMP) is a promising technique for the accurate detection of pathogens in organisms and the environment. Here we present a comparative study of the performance of six fluorescent intercalating dyes-SYTO-9, SYTO-13, SYTO-82, SYBR Green I, SYBR Gold, EvaGreen-in three different qLAMP model systems. SYTO-9 and SYTO-82, which had the best results, were used for additional enzyme and template titration studies. SYTO-82 demonstrated the best combination of time-to-threshold (Tt) and signal-to-noise ratio (SNR).
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