Journal
TALANTA
Volume 204, Issue -, Pages 762-768Publisher
ELSEVIER
DOI: 10.1016/j.talanta.2019.06.064
Keywords
Two-photon fluorescence; Cysteine; Lysosome; Probe; Ultralow concentration
Categories
Funding
- National Natural Science Foundation of China [51703171, 21525523, 21722507]
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Herein we reported a two-photon (TP) fluorescence turn-on probe MNPO, exhibiting high selectivity and sensitivity towards intracellular cysteine (Cys) with excellent lysosomal localization. The probe displayed fast response towards Cys over homocysteine (Hcy), glutathione (GSH), and other various analytes under physiological conditions. Low cytotoxicity made it successful for TP imaging of Cys in HeLa cells with an ultralow probe concentration of 250 nM, and a rapid response of only 10 min. Simultaneously, colocalization experiments in lysosome demonstrated its ability for specific in situ detection of lysosomal Cys in living cells, which shed light on its potential applications in biomedical applications. Beyond that MNPO was successfully applied for TP imaging of Cys in mice organ tissues such as heart, liver, and spleen, and the penetration depth of mice heart tissue was up to 184 mu m, which disclosed the predominant TP characteristic. We believe that this study will provide some useful information toward diagnosis and treatment of pathogenesis associated with Cys or lysosomes in future.
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