4.7 Article

Molecular imprinted electrospun chromogenic membrane for L-tyrosine specific recognition and visualized detection

Journal

TALANTA
Volume 204, Issue -, Pages 647-654

Publisher

ELSEVIER
DOI: 10.1016/j.talanta.2019.06.051

Keywords

Molecular imprinting; Chromogenic; Electrospinning; PVA; Ninhydrin; Visualization

Funding

  1. Fujian Province Natural Science Foundation, University Joint Funded Project in 2017, China [2017J01416]
  2. Fujian Province Higher Education and Teaching Reform Project, China [FBJG20180080]
  3. Fuzhou University

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In this work, molecularly imprinted electrospun chromogenic membrane(MIP-ECM) was synthesized using P-Hydroxybenzene propanoic acid as imitate template molecule and Poly (vinyl alcohol)(PVA) as substrate. Ninhydrin is used as chromogenic agent for visualizing detection of target molecule L-tyrosine(Tyr). The results showed that molecularly imprinted membrane exhibited selectivity and specific adsorption capacity for L-tyrosine. The influences of environmental factors, such as reaction temperature, the amount of ninhydrin and elution time, on chromogenic properties were examined by SEM and optical microscope. Gray value analysis is proceeded by software Image J. Due to the facilitate preparation and intuitive visualization, the proposed method was a promising candidate for selective recognition of bio-generated acid and amino molecules.

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