Journal
SPECTROCHIMICA ACTA PART A-MOLECULAR AND BIOMOLECULAR SPECTROSCOPY
Volume 222, Issue -, Pages -Publisher
PERGAMON-ELSEVIER SCIENCE LTD
DOI: 10.1016/j.saa.2019.117262
Keywords
TBT-NBD; Fluorescent probe; Cys/Hcy; Cell imaging
Categories
Funding
- National Natural Science Foundation of China [21601111, 21475080]
- Shanxi Scholarship Council of China [2017-011]
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A fluorescent probe, 4-(benzothiazole-2-ylthio)-7-nitro-2,1,3-benzoxadiazole (TBT-NBD) was developed for cysteine (Cys) and homocysteine (Hcy). The reaction mechanism was based on the Cys/Hcy-induced nucleophilic substitution of benzothiazole thioether then Smiles rearrangement reaction to form corresponding amino-nitrobenzoxadiazole, which emitted yellow-green fluorescence and guaranteed the high selectivity for Cys/Hcy over glutathione (GSH). TBT-NBD could detect Cys/Hcy within 5 min in the presence of high concentration of GSH and other amino acids. Moreover, TBT-NBD had been exploited to identify intracellular Cys/Hcy in living cells in light of its low toxicity. (C) 2019 Published by Elsevier B.V.
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