Journal
BIOSENSORS & BIOELECTRONICS
Volume 80, Issue -, Pages 519-524Publisher
ELSEVIER ADVANCED TECHNOLOGY
DOI: 10.1016/j.bios.2016.02.018
Keywords
Cancer; Graphene oxide; Drug release; Therapeutic monitoring; Caspase-3
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Funding
- National Natural Science Foundation of China [21505161]
- Natural Science Foundation of Jiangsu Province [BK20150701]
- Program for Changjiang Scholars and Innovative Research Team in University [IRT1193]
- State Key Laboratory of Analytical Chemistry for Life Science [SKLACLS1415]
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This work reports a graphene oxide-based nanovehicle with conjugation of pegylated folate for targeted delivery of anticancer drugs and fluorescein-labeled peptide for therapeutic self-monitoring in vitro and in vivo. The nanovehicle could absorb hydrophobic and aromatic drug molecules with high loading capacity and efficiency of more than 1.7 mg mg(-1) and 90%, respectively. MIT and flow cytometric assays demonstrated that the drug-loaded nanovehicle could specifically transport and release the drugs into the folate receptor high-expressed cancer cells, which ensured a high therapeutic efficiency to cancer cells and prevented the injury to normal cells. Moreover, confocal fluorescence imaging confirmed that the drug-induced cancer cell death could be visualized with the light-up fluorescence of fluorescein activated by caspase-3. The targeted delivery of drug and self-evaluation of therapeutic efficacy were further successfully realized by living imaging in tumor-bearing mice, which broaden the applications of this theranostic system in vivo and may offer new opportunities for precise cancer treatment. (C) 2016 Elsevier B.V. All rights reserved.
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