Journal
BIOSENSORS & BIOELECTRONICS
Volume 77, Issue -, Pages 666-672Publisher
ELSEVIER ADVANCED TECHNOLOGY
DOI: 10.1016/j.bios.2015.10.046
Keywords
Gold nanoclusters; Alkaline phosphatase; Immunoassay; Fluorescent biosensing
Categories
Funding
- National Natural Science Foundation of China [21275065]
- Fundamental Research Funds for the Central Universities [JUSRP51314B]
- Opening Foundation of the State Key Laboratory of Analytical Chemistry for Life Science of Nanjing University [KLACLS1008]
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In this work, a novel strategy for modulating the fluorescence of gold nanoclusters (Au NCs) is developed. The fluorescence of bovine serum albumin (BSA) protected Au NCs is firstly quenched by KMnO4 and then restored by ascorbic acid (AA) due to the deterioration/restoration of the surface structure. Based on which, a novel switch-on fluorescent assay for probing the activity of alkaline phosphatase (ALP) is developed with a detection limit as low as 0.002 U/L. In addition, this testing protocol is also expanded to the detection of the inhibitor of ALP and mouse IgG (as a model), the detection limits are 15 ng/mL for the inhibitor of 2,4-Dichlorophenoxyacetic acid (2,4-DA) and 1.5 pg/mL for mouse IgG. The present method paves a new way to develop convenient, sensitive, and selective metal NCs-based fluorescent turn-on probes with promising applications in versatile biosensing. (C) 2015 Elsevier B.V. All rights reserved.
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