Journal
BIOSENSORS & BIOELECTRONICS
Volume 79, Issue -, Pages 379-385Publisher
ELSEVIER ADVANCED TECHNOLOGY
DOI: 10.1016/j.bios.2015.12.080
Keywords
Cathodic electrochemiluminescence; Metal nanoparticle; Cancer biomarker; Metal-organic framework
Categories
Funding
- Natural Science Foundation of China [21175057, 21375047, 21377046, 21505051]
- Science and Technology Plan Project of Jinan [201307010]
- Science and Technology Development Plan of Shandong Province [2014GSF120004]
- Special Project for Independent Innovation and Achievements Transformation of Shandong Province [2014ZZCX05101]
- Special Foundation for Taishan Scholar Professorship of Shandong Province
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In this work, silver nanoparticles-doped Pb (II) metal-organic framework (Ag-MOF) was prepared and exploited as a luminescence probe for the development of label-free electrochemiluminescence (ECL) immunosensing scheme for prostate-specific antigen (PSA). The P-cyclodextrin based MOF, Pb-beta-cyclodextrin (Pb(II)-beta-CD) shows excellent ECL behavior and unexpected reducing capacity towards silver ions. Silver nanoparticles could massively form on the surface of Pb(II)-beta-CD (Ag@Pb(II)-beta-CD) without use any additional reducing agent, while the ECL behavior of Pb(II)-beta-CD still was well retained. The Ag@Pb(II)-beta-CD was used as a substrate material to modify glass carbon electrodes and formed a sensing platform for the fabricating ECL immunosensor. The presence of silver nanoparticles enables the facile immobilization of capturing antibody of PSA. The specific binding of PSA onto the electrode surface induces the decrease of ECL signals. A linear range of 0.001-50 ng mL(-1) with a detection limit of 0.34 pg mL(-1) (S/N=3) was obtained after the optimization of experimental conditions. This simply fabricated immunosensor exhibits good stability, accuracy and acceptable reproducibility, which suggesting its potential applications in clinical diagnostics. (C) 2015 Elsevier B.V. All rights reserved.
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