Journal
BIOSENSORS & BIOELECTRONICS
Volume 80, Issue -, Pages 265-272Publisher
ELSEVIER ADVANCED TECHNOLOGY
DOI: 10.1016/j.bios.2016.01.070
Keywords
Fluorescent biosensor; Functional nucleic acids; Heavy metal ions; On-site; Regeneration
Categories
Funding
- National Natural Science Foundation of China [21377065]
- National Key Scientific Instrument and Equipment Development Plan [2012YQ030111]
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A turn-on evanescent-wave fiber biosensor based on functional nucleic acids was developed for on-site detection of Hg2+ and Pb2+ in environmental water samples. A two-step strategy was conducted to take advantage of the rapid binding of T-T mismatches with Hg2+ and the cleavage of a substrate by 8-17 DNAzyme with Pb2+ in solution, as well as sensitive DNA detection on a liquid-solid interface based on evanescent wave. The biosensor demonstrated high sensitivity (with a detection limit of 22 pM for Hg2+ and 20 nM for Pb2+) and selectivity without any signal amplification, rapid detection (within 13 min per test), low cost (10-20 Chinese yuan per sample), and multiple-cycle regeneration (at least 18 times). The method was also successfully applied on Hg2+ and Pb2+ detection in real environmental water samples. (C) 2016 Elsevier B.V. All rights reserved.
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