Journal
SEPARATION AND PURIFICATION TECHNOLOGY
Volume 224, Issue -, Pages 95-105Publisher
ELSEVIER SCIENCE BV
DOI: 10.1016/j.seppur.2019.05.002
Keywords
Hybrid composite macroporous structure; Polymer brush; Clickablephenylboronic acid; Atom transfer radical polymerization; Bioseparation
Categories
Funding
- Swedish Foundation for Strategic Research [2015-491]
- Swedish Research Council FORMAS [2016-00591]
- Formas [2016-00591] Funding Source: Formas
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The formation of a hybrid composite material consisted of polymer brushes and phenylboronic acid ligands on a macroporous cryogel is reported here for the first time for effective adsorption of glycoprotein and yeast cells. The surface area of the cryogel was increased by covalent immobilization of silica nanoparticles. Random co-polymer brushes composed of N-isopropylacrylamide and glycidyl methacrylate were grown on the surface of the nanoparticles using surface-initiated atom transfer radical polymerization. Boronic acid ligands were attached to the polymer brush via Cu(I)-catalysed azide-alkyne cycloaddition click reaction. Due to the macro-porosity of the final affinity material, particulate-containing fluids can pass through the composite cryogel at a high flow rate with almost no back pressure. An affinity column packed with the composite cryogel was tested to evaluate the capability of selective protein and cell separation. Glycated haemoglobin and yeast cells were successfully separated from a complex protein mixture and from a wastewater sample, respectively. The dynamic binding capacity of the column was found to be 63.9 and 6 mg/g gel for haemoglobin and yeast cells, respectively.
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