Journal
BIOSENSORS & BIOELECTRONICS
Volume 80, Issue -, Pages 433-441Publisher
ELSEVIER ADVANCED TECHNOLOGY
DOI: 10.1016/j.bios.2016.01.092
Keywords
Molecular imprinting; Gold nanorods; Nanosensor; Surface-enhanced Raman scattering; Protein biomarker
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Funding
- National Natural Science Foundation of China [21576017, 21436002, 21306006]
- 973 programs [2014CB745103, 2013CB733603]
- 863 programs [2015BAD15B07]
- Xiamen Scientific and Technological project [3502Z20142012]
- Public Hatching Platform for Recruited Talents of Beijing University of Chemical Technology
- Fundamental Research Funds for the Central Universities [YS1407, buctrc201413]
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Molecularly imprinted plasmonic nanosensor has been prepared via the rational design of an ultrathin polymer layer on the surface of gold nanorods imprinted with the target protein. This nanosensor enabled selective fishing-out of the target protein biomarker even from a complex real sample such as human serum. Sensitive SERS detection of the protein biomarkers with a strong Raman enhancement was achieved by formation of protein imprinted gold nanorods aggregates, stacking of protein imprinted gold nanorods onto a glass plate, or self-assembly of protein imprinted gold nanorods into close-packed array. High specificity and sensitivity of this method were demonstrated with a detection limit of at least 10(-8) mol/L. for the target protein. This could provide a promising alternative for the currently used immunoassays and fluorescence detection, and offer an ultrasensitive, non-destructive, and label-free technique for clinical diagnosis applications. (C) 2016 Elsevier B.V. All rights reserved.
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