Journal
BIOSENSORS & BIOELECTRONICS
Volume 79, Issue -, Pages 822-828Publisher
ELSEVIER ADVANCED TECHNOLOGY
DOI: 10.1016/j.bios.2016.01.022
Keywords
Carbon dots; Cell imaging; Adenosine-5'-triphosphate (ATP); Fluorescent probe; Reactive oxygen species (ROS) and reactive nitrogen species (RNS); Real-time measurement
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Funding
- National Natural Science Foundation of China [21275018, 21203008]
- Beijing National Laboratory for Molecular Sciences (BNLMS) [20140121]
- Research Fund for the Doctoral Program of Higher Education of China (RFDP) [20121101110049]
- 111 Project [B07012]
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Phosphorus and nitrogen doped carbon dots (PN-CDs) were conveniently prepared by carbonization of adenosine-5'-triphosphate using a hydrothermal treatment. The PN-CDs with P/C atomic ratio of ca. 9.2/100 emit blue luminescence with high quantum yields of up to 23.5%. The PN-CDs were used as a novel sensing platform for live cell imaging of reactive oxygen species (ROS) and reactive nitrogen species (RNS), including ClO-, ONOO-, and NO in macrophages. The nanosensor design is based on our new finding that the strong fluorescence of the PN-CDs can be sensitively and selectively quenched by ROS and RNS both in vitro and in vivo. These results reveal that the PN-CDs can serve as a sensitive sensor for rapid imaging of ROS and RNS signaling with high selectivity and contrast. (C) 2016 Elsevier B.V. All rights reserved.
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