Journal
BIOSENSORS & BIOELECTRONICS
Volume 79, Issue -, Pages 411-415Publisher
ELSEVIER ADVANCED TECHNOLOGY
DOI: 10.1016/j.bios.2015.12.081
Keywords
Mercury(II) ion; DNA-scaffolded silver nanocluster; Exonuclease III-assisted target recycling amplification; Fluorescent sensing platform
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Funding
- National Natural Science Foundation of China [41176079, 21475025]
- National Science Foundation of Fujian Province [2014J07001]
- Key Science Project (Type A) of the Fujian Provincial Department of Education, China [JA12021]
- Program for Changjiang Scholars and Innovative Research Team in University [IRT1116]
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A new label-free DNA sensing protocol was designed for fluorescent detection of mercury(II) (Hg2+), coupling hairpin DNA-scaffolded silver nanocluster (DNA-AgNC) with exonuclease III-assisted target recycling amplification. The assay was carried out through target-induced conformational change of hairpin DNA, while the signal derived from the formed silver nanoclusters on hairpin DNA probes. Initially, target Hg2+ was specifically coordinated with thymine-thymine (T-T) mismatches to form an intact hairpin DNA. Then, the newly formed hairpin DNA was digested through exonuclease III from blunt 3' termini and restrained at 3' protruding terminus, thus resulting in the release of target Hg2+ from hairpin DNA. The liberated target Hg2+ initiated the next cycling, thereby causing the conformational change of numerous hairpin probes from the stem-loop DNA structure to single-stranded DNA. Under the optimal conditions, the fluorescent intensity of the as-produced DNA-AgNCs decreased with the increasing Hg2+ concentration within a dynamic range from 0.1 nM to 10 nM with a detection limit (LOD) of 24 pM. Moreover, the low-cost fluorescent sensing system exhibited high reproducibility and good specificity, thus representing an optional sensing platform for rapid screening of Hg2+ in environmental water samples. (C) 2015 Elsevier B.V. All rights reserved.
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