Journal
BIOSENSORS & BIOELECTRONICS
Volume 75, Issue -, Pages 446-451Publisher
ELSEVIER ADVANCED TECHNOLOGY
DOI: 10.1016/j.bios.2015.09.002
Keywords
SERS; Fluorescence; Carbon nanotubes; Dox; pH sensor
Categories
Funding
- National Key Basic Research Program of China [2015CB352002]
- Excellent Youth Foundation of Jiangsu Province [BK20140023]
- National Natural Science Foundation of China (NSFC) [61535003, 61275182, 61177033, 61505027]
- Innovation Fund of School of Electronic Science and Engineering, Southeast University [2242015KD002]
- Science Foundation for The Excellent Youth Scholars of Southeast University
- Scientific Research Foundation of Graduate School of Southeast University
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We fabricate a multifunctional nanocarrier based on multi-walled carbon nanotubes (MWCNTs) decorated with gold/silver core-shell nanoparticles (Au@Ag NPs) and fluorescein isothiocyanate (FITC) for tracking the intracellular drug release process. In the demonstrated nanocarrier, the Au@Ag NPs adsorbed on the surface of MWCNTs were labeled with the pH-dependent SERS reporter 4-Mercapto-benzoic acid (4MBA) for SERS based pH sensing. FITC was conjugated on MWCNTs to provide fluorescence signal for tracing the MWCNTs. Fluorescent doxorubicin (DOX) was used as the model drug which can be loaded onto MWCNTs via pi-pi stacking and released from the MWCNTs under acidic condition. By detecting the SERS spectrum of 4MBA, the pH value around the nanocarrier could be monitored. Besides, by tracing the fluorescence of FITC and DOX, we can also investigate the drug release process in cells. Experimental results show that the proposed nanocarrier retained a well pH-sensitive performance in living cells, and the DOX detached from MWCNTs inside the lysosomes and entered into the cytoplasm with the MWCNTs being left in lysosomes. To further investigate the drug release dynamics, 2-D color-gradient pH mapping were plotted, which were calculated from the SERS spectra of 4MBA. The detailed release process and carrier distribution have been recorded as environmental pH changes during cell endocytosis. Furthermore, we also confirmed that the proposed nanocarrier has a good biocompatibility. It indicates that the designed nanocarrier have a great potential in intraceable drug delivery, cancer cells imaging and pH monitoring. (C) 2015 Elsevier B.V. All rights reserved.
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