Journal
BIOSENSORS & BIOELECTRONICS
Volume 85, Issue -, Pages 751-757Publisher
ELSEVIER ADVANCED TECHNOLOGY
DOI: 10.1016/j.bios.2016.05.073
Keywords
Micromechanical biosensor; Human stem cell; Cardiomyocyte contraction; Drug testing
Categories
Funding
- CEITEC - Central European Institute of Technology [CZ.1.05/1.1.00/02.0068, LQ1601]
- FNUSA-ICRC from European Regional Development Fund [CZ.1.05/1.1.00/ 02.0123, LQ1605]
- National Program of Sustainability II., Grant Agency of the Czech Republic [P302/12/G157, GA13-19910S]
- SoMoPro - Marie Curie Actions - South Moravian Region
- European Society of Cardiology (ESC) [R12042FF]
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Cardiomyocyte contraction and relaxation are important parameters of cardiac function altered in many heart pathologies. Biosensing of these parameters represents an important tool in drug development and disease modeling. Human embryonic stem cells and especially patient specific induced pluripotent stem cell-derived cardiomyocytes are well established as cardiac disease model.. Here, a live stem cell derived embryoid body (EB) based cardiac cell syncytium served as a biorecognition element coupled to the microcantilever probe from atomic force microscope thus providing reliable micromechanical cellular biosensor suitable for whole-day testing. The biosensor was optimized regarding the type of cantilever, temperature and exchange of media; in combination with standardized protocol, it allowed testing of compounds and conditions affecting the biomechanical properties of EB. The studied effectors included calcium, drugs modulating the catecholaminergic fight-or-flight stress response such as the beta-adrenergic blocker metoprolol and the beta-adrenergic agonist isoproterenol. Arrhythmogenic effects were studied using caffeine. Furthermore, with EBs originating from patient's stem cells, this biosensor can help to characterize heart diseases such as dystrophies. (C) 2016 Elsevier B.V. All rights reserved.
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