Journal
BIOSENSORS & BIOELECTRONICS
Volume 82, Issue -, Pages 185-194Publisher
ELSEVIER ADVANCED TECHNOLOGY
DOI: 10.1016/j.bios.2016.04.004
Keywords
Non-enzymatic sensor; Graphene; Hydrogen peroxide; Ferrocene carboxylic acid; Platinum nanoparticles
Categories
Funding
- National Natural Science Foundation of China [21265003, 81406451]
- Program for New Century Excellent Talents in University of Chinese Ministry of Education [NCET-13-0744]
- Postdoctoral Research Fund of China [2013T60830]
- Fund of Guangxi Key laboratory of Metabolic Diseases Research [2015-181 h-02, 2015-181 h-03]
- Innovation Project of Guangxi Graduate Education [YCSZ2015151]
- Project of outstanding young teachers' training in higher education institutions of Guangxi
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A highly sensitive non-enzymatic electrochemical sensor based on platinum nanoparticles/reduced graphene oxide-chitosan-ferrocene carboxylic acid nano-hybrids (Pt NPs/RGO-CS-Fc biosensor) was developed for the measurement of hydrogen peroxide (H2O2). The RGO-CS-Fc nano-hybrids was prepared and characterized by UV-vis spectrum, Fourier transform infrared spectroscopy, transmission electron microscopy, Raman spectrometer and electrochemical impedance spectroscopy. Under optimal experimental conditions, the Pt NPs/RGO-CS-Fc biosensor showed outstanding catalytic activity toward H2O2 reduction. The current response of the biosensor presented a linear relationship with H2O2 concentration from 2.0 x 10(-8) M to 3.0 x 10(-8) M with a correlation coefficient of R-2=0.9968 and with logarithm of H2O2 concentration from 6.0 x 10(-6) M to 1.0 x 10(-2) M with a correlation coefficient of R-2=0.9887, the low detection limit of 20 nM was obtained at the signal/noise (SAN) ratio of 3. Moreover, the Pt NPs/RGO-CS-Fc biosensor exhibited excellent anti-interference capability and reproducibility for the detection of H2O2. The biosensor was also successfully applied for the detection of H2O2 from living cells containing normal and cancer cells. All these results prove that the Pt NPs/RGO-CS-Fc biosensor has the potential application in clinical diagnostics to evaluate oxidative stress of different living cells. (C) 2016 Elsevier B.V. All rights reserved.
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