Journal
BIOSENSORS & BIOELECTRONICS
Volume 75, Issue -, Pages 1-7Publisher
ELSEVIER ADVANCED TECHNOLOGY
DOI: 10.1016/j.bios.2015.08.007
Keywords
meso-(4-pyridinyl)-substituted BODIPY dyes; Gold-nanoparticle based sensor; Thiols; Turn-on fluorescent response; In Vitro and in vivo imaging
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Funding
- National Natural Science Foundation of China [20972170, 21275150]
- Funds for Distinguished Young Scientists of Gansu [1210RJDA013]
- Natural Science Foundation of Gansu province [1107RJYA069]
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A novel gold nanoparticle (AuNP)-based sensor for detecting thiols in aqueous solution has been developed. Due to the weak N center dot center dot center dot Au interactions, meso-(4-pyridiny1)-substituted BODIPY (4,4-difluoro-4-bora-3a,4a-diaza-s-indacene) dyes were coordinated to AuNP surfaces, which effectively quenched the fluorescence of organic/inorganic hybrid systems. The fluorescent quenching mechanism was mainly ascribed to the highly efficient fluorescent resonance energy transfer (FRET) and the inner filter effect. In the presence of thiols, meso-(4-pyridinyl)-substituted BODIPY chromophore were displaced and released from the AuNP surfaces and thus restored the fluorescence of BODIPY chromophore. The modulation of the fluorescence quenching efficiency of BODIPY-AuNPs in the presence of thiols can achieve a large turn-on fluorescence enhancement (40-fold) in aqueous solution. The new AuNP-based fluorescence sensor displayed desired properties such as high specificity, relatively low detection limit (30 nM for Cys), appreciable water solubility and rapid response time (within 2 min for Cys/Hcy). Moreover, the sensor has been successfully applied for monitoring and imaging of intracellular thiols within living HeLa cells. (C) 2015 Elsevier B.V. All rights reserved.
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