Journal
BIOSENSORS & BIOELECTRONICS
Volume 86, Issue -, Pages 330-336Publisher
ELSEVIER ADVANCED TECHNOLOGY
DOI: 10.1016/j.bios.2016.05.101
Keywords
Molecularly imprinted polymers; Myoglobin; Poly-o-phenylenediamine; Electropolymerization
Categories
Funding
- Ministry of Science and Education of Russian Federation [14.576.21.0045, RFMEF157614X0045]
Ask authors/readers for more resources
Electrosynthesis of molecularly imprinted polymer (MIP) templated with myoglobin (Mb) and the reference non-imprinted polymer (NIP) was examined with o-phenylenediamine (o-PD) as a monomer. Mass-sensitive quartz crystal microbalance with dissipation monitoring supplied by an electrochemical module (EQCM-D) was applied to characterize and optimize MIP/NIP electrosynthesis. Mb rebinding was detected by direct electrocatalytic reduction of Mb by square wave voltammetry (SWV) or differential pulse voltammetry (DPV). The results obtained showed high specificity of polymeric antibodies to template Mb, with an imprinting factor determined as a ratio I-max(MIP)/I-max (NIP) of 2-4. The prepared MIP sensor is characterized by an apparent dissociation constant of (3.3 +/- 0.5) x 10(-9) M and has a broad range of working concentrations of 1 nM-1 mu M, with the detection limit of 0.5 mu M (9 ng/ml). Mb rebinding was examined in Mb-free diluted human serum spiked with Mb as well as in plasma samples of patients with acute myocardial infarction (AMI) and in control plasma of healthy donors in order to demonstrate the potential medical application of developed MIP sensors. (C) 2016 Elsevier B.V. All rights reserved.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available