Journal
BIOSENSORS & BIOELECTRONICS
Volume 77, Issue -, Pages 565-572Publisher
ELSEVIER ADVANCED TECHNOLOGY
DOI: 10.1016/j.bios.2015.10.013
Keywords
Neopterin; Potentiometric chemosensor; Molecularly imprinted polymer; Open-circuit potential; Cancer biomarker
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Funding
- Polish National Science Centre (NCN) [2011/03/D/ST4/02596, 2014/15/B/ST4/04642, 2014/15/B/NZ7/01011]
- US National Science Foundation [1401188]
- Foundation for Polish Science through the International PhD Project Program - European Regional Development Fund within the Innovative Economy Operational Program Grants for Innovations, Fondazione Cariplo [2011-0417, MPD/2009/1/styp15]
- Direct For Mathematical & Physical Scien
- Division Of Chemistry [1401188] Funding Source: National Science Foundation
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With an established procedure of molecular imprinting, a synthetic polymer receptor for the neopterin cancer biomarker was devised and used as a recognition unit of a potentiometric chemosensor. For that, bis-bithiophene derivatized with cytosine and bithiophene derivatized with boronic acid were used as functional monomers. The open-circuit potential (OCP) based transduction under flow-injection analysis conditions (FIA) determined neopterin in the concentration range of 0.15-2.5 mM with the 22 mu M limit of detection (LOD) and 7.01(+/- 0.15) mV mM(-1) sensitivity indicating its potential suitability in clinical analysis applications. The molecularly imprinted polymer (MIP) film showed an appreciable apparent imprinting factor of similar to 6. The chemosensor successfully discriminated the interferences including the 6-biopterin and pterin structural analogs of neopterin as well as glucose and creatinine. Moreover, it determined neopterin in synthetic serum samples. (C) 2015 Elsevier B.V. All rights reserved.
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