Journal
BIOSENSORS & BIOELECTRONICS
Volume 78, Issue -, Pages 67-72Publisher
ELSEVIER ADVANCED TECHNOLOGY
DOI: 10.1016/j.bios.2015.11.011
Keywords
Chloramphenicol; Core-shell nanostructure; Aptamer; SERS; Cy5 dye
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Funding
- Fundamental Research Funds for the Central Universities [0806J0452]
- National Science & Technology Pillar Program [2015BAD16B00]
- International Technology Cooperation Program of Jiangsu province, China [BZ2014034]
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In this study, we described a novel method for highly sensitive and specific detection of chloramphenicol (CAP) based on engineered hot Au core-Ag shell nanostructures (Au@Ag NSs). Cy5-labeled DNA aptamer was embedded between the Au and Ag layers as a signal generator and target-recognition element, to fabricate uniform Au@Ag NSs with unexpected strong and stable SERS signals. The presented CAP can specifically bind to the DNA aptamer by forming an aptamer-CAP conjugate, and cause greatly decreased SERS signals of Au@Ag NSs. By using this method, we were able to detect as low as 0.19 pg mL(-1) of CAP with high selectivity, which is much lower than those previously reported biosensors. Compared with the other SERS sensors that attached a dye in the outer layer of nanoparticles, this method exhibits excellent sensitivity and has the potential to significantly improve stability and reproducibility of SERS-based detection techniques. (C) 2015 Elsevier B.V. All rights reserved.
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