Journal
BIOSENSORS & BIOELECTRONICS
Volume 77, Issue -, Pages 681-686Publisher
ELSEVIER ADVANCED TECHNOLOGY
DOI: 10.1016/j.bios.2015.10.044
Keywords
Mercury(II) ion; Invertase-functionalized gold-dendrimer nanospheres; Thymine-Hg2+-thymine; Personal glucometer
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Funding
- National Natural Science Foundation of China [41176079, 21475025]
- National Science Foundation of Fujian Province [2014J07001]
- Key Science Project (type A) of the Department of Education, Fujian Province, China [JA12021]
- Program for Changjiang Scholars and Innovative Research Team in University [IRT1116]
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A simple, low-cost transducer with glucometer readout was designed for sensitive detection of mercury (II) (Hg2+), coupling with thymine-Hg2+-thymine (T-Hg2+-T) coordination chemistry and invertasefunctionalized gold-dendrimer nanospheres for the signal amplification. Initially, nanogold-encapsulated poly(amidoamine) dendrimers (Au DENs) were synthesized by in-situ reduction of gold(III). Thereafter, the as-prepared Au DENs were utilized for the labeling of invertase and T-rich signal DNA probe. In the presence of target Hg2+, the functionalized Au DENs were conjugated to capture DNA probe-modified electrode via T-Hg2+-T coordination chemistry. Accompanying the Au DENs, the labeled invertase could hydrolyze sucrose into glucose, which could be quantitatively monitored by an external personal glucometer (PGM). The PGM signal increased with the increasing target Hg2+ in the sample. Under the optimal conditions, our designed sensing platform exhibited good PGM responses toward target Hg2+, and allowed the detection of Hg2+ at a concentration as low as 4.2 pM. This sensing system also displayed remarkable specificity relative to target Hg2+ against other competing ions, and could be applied for reliable monitoring of spiked Hg2+ into the environmental water samples with satisfactory results. With the advantages of cost-effectiveness, simplicity, portability, and convenience, our strategy provides a tremendous potential to be a promising candidate for point-of-use monitoring of non-glucose targets by the public. (C) 2015 Elsevier B.V. All rights reserved.
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