Journal
SCIENCE OF THE TOTAL ENVIRONMENT
Volume 687, Issue -, Pages 702-711Publisher
ELSEVIER
DOI: 10.1016/j.scitotenv.2019.06.047
Keywords
Hypoxia exposure; Cu acclimation; Oxidative stress; Nrf2; HIF-1 alpha
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Funding
- Natural Science Foundation of Zhejiang Province [LY18D060008]
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The aim of the present study was to evaluate the effects of Cu pre-exposure on antioxidant defense and energy metabolism in the liver of the large yellow croaker exposed to severe hypoxia. Fish were pre-acclimated to 0 and 30 mu g Cu L-1 for 96 h, and subsequently exposed to 7.0 and 1.5 mg DO L-1 for another 24 h. Hypoxic stress alone increased reactive oxygen species and hepatic vacuoles. When compared to hypoxic stress alone, hypoxic stress plus Cu pre-exposure increased mortality and ROS production, and worsened histological structure by inhibiting antioxidant defense and aerobic metabolism, and enhancing anaerobic metabolism, suggesting Cu pre-acclimation aggravated hypoxia-induced oxidative damage. NFE2-related nuclear factor 2 and hypoxia-inducible factor-1 alpha might participate in the transcriptional regulation of genes related to antioxidant response and energymetabolism, respectively. In conclusion, Cu pre-acclimation had a synergistic effect on antioxidant response and energy metabolismin fish under severe hypoxia, which contributes to understanding the molecular mechanisms underlying negative effects of Cu pre-acclimation against hypoxic damage in fish. (C) 2019 Elsevier B.V. All rights reserved.
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