Journal
SCIENCE OF THE TOTAL ENVIRONMENT
Volume 688, Issue -, Pages 917-925Publisher
ELSEVIER
DOI: 10.1016/j.scitotenv.2019.06.364
Keywords
Resuscitation promoting factor; Resuscitated strain; PCB 77; Castellaniella sp.; Degradation capability
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Funding
- National Natural Science Foundation of China [41701354, 51808501]
- Natural Science Foundation of Zhejiang Province, China [LQ17D010002, LQ18E080008]
- Project of Jinhua Science and Technology Bureau, China [2018-4-007, 2018-4-005]
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Resuscitated strains which were obtained by addition of resuscitation promoting factor (Rpf) could provide a vast majority of microbial source for obtaining highly efficient polychlorinated biphenyl (PCB)-degrading bacteria. In this study, the Caslellaniella sp. strain SPC4 which was resuscitated by Rpf addition showed the highest efficiency in degradation of 3,3',4,4'-tetrachlorobiphenyl (PCB 77) among the resuscitated and non-resuscitated isolates. Further investigations on the PCB 77 degradation capability of the resuscitated strain SPC4 showed that SPC4 could efficiently degrade PCB 77 with maximum degradation rate (q(max)) of 0.066/h at about 20 mg/L of PCB 77. The maximum growth rate on PCB 77 was 2.663 x 10(7) CFU/(mL.h) (0.024/h). The most suitable model of Edward demonstrated that the SPC4 could achieve q(max) of 0.9315/h, with substrate-affinity of 11.33 mg/L and substrate-inhibition constants of 11.41 mg/L. Meanwhile, the presence of bphA gene expression and chlorine ions release, together with the identification of metabolites, confirmed that the bph-encoded biphenyl pathway was involved in KB 77 mineralization by SPC4. This report is the first to demonstrate aerobic degradation of PCB 77 by the resuscitated strain Castellaniella sp. SPC4, indicating excellent potential for PCB bioremediation. (C) 2019 Elsevier B.V. All rights reserved.
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