Journal
BIOSENSORS & BIOELECTRONICS
Volume 81, Issue -, Pages 39-45Publisher
ELSEVIER ADVANCED TECHNOLOGY
DOI: 10.1016/j.bios.2016.01.095
Keywords
Agricultural biosensor; Bi-enzyme electrode; Methyl salicylate; Alcohol oxidase; Horseradish peroxidase
Categories
Funding
- National Science Foundation [CBET-1159540]
- ACS Herman Frasch Foundation [045097-01]
- National Institute of Food and Agriculture [2015-67021-23188]
- Div Of Chem, Bioeng, Env, & Transp Sys
- Directorate For Engineering [1159540] Funding Source: National Science Foundation
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An amperometric sensor based on a bi-enzyme modified electrode was fabricated to detect methyl salicylate, a volatile organic compound released by pathogen-infected plants via systemic response. The detection is based on cascadic conversion reactions that result in an amperometric electrochemical signal. The bi-enzyme electrode is made of alcohol oxidase and horseradish peroxidase enzymes immobilized on to a carbon nanotube matrix through a molecular tethering method. Methyl salicylate undergoes hydrolysis to form methanol, which is consumed by alcohol oxidase to form formaldehyde while simultaneously reducing oxygen to hydrogen peroxide. The hydrogen peroxide will be further reduced to water by horseradish peroxidase, which results in an amperometric signal via direct electron transfer. The bi-enzyme biosensor was evaluated by cyclic voltammetry and constant potential amperometry using hydrolyzed methyl salicylate as the analyte. The sensitivity of the bi-enzyme biosensor as determined by cyclic voltammetry and constant potential amperometry were 112.37 and 282.82 mu A cm(-2) mM(-1) respectively, and the corresponding limits of detection were 22.95 and 0.98 mu M respectively. Constant potential amperometry was also used to evaluate durability, repeatability and interference from other compounds. Wintergreen oil was used for real sample study to establish the application of the bi-enzyme sensor for selective determination of plant pathogen infections. (C) 2016 Elsevier B.V. All rights reserved.
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