Journal
BIOSENSORS & BIOELECTRONICS
Volume 83, Issue -, Pages 361-367Publisher
ELSEVIER ADVANCED TECHNOLOGY
DOI: 10.1016/j.bios.2016.04.057
Keywords
Reduced graphene oxide; Yttria; Nanocomposite; Electrochemical genosensor; BRCA1 gene; Gold nanoparticle cluster; Attomolar detection
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Funding
- Council of Scientific and Industrial Research [01(2762)/13/EMR-II]
- Department of Science and Technology, Government of India [SR/FTP/CS-28/2006]
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Reduced graphene oxide-yttria nanocomposite (rGO:Y) is applied as electrochemical genosensor platform for ultrahigh sensitive detection of breast cancer 1 (BRCA1) gene for the first time. The sensor is based on the sandwich assay in which gold nanoparticle cluster labeled reporter DNA hybridize to the target DNA. Glassy carbon electrode modified with rGO-yttria serves as the immobilization platform for capture probe DNA. The sensor exhibited a fine capability of sensing BRCA1 gene with linear range of 10 attomolar (aM) to 1 nanomolar (nM) and a detection limit of 5.95 attomolar. The minimum distinguishable response concentration is down to the attomolar level with a high sensitivity and selectivity. We demonstrated that the use of rGO:Y modified electrode along with gold nanoparticle cluster (AuNPC) label leads to the highly sensitive electrochemical detection of BRCA1 gene. (C) 2016 Elsevier B.V. All rights reserved.
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