Journal
BIOSENSORS & BIOELECTRONICS
Volume 79, Issue -, Pages 71-78Publisher
ELSEVIER ADVANCED TECHNOLOGY
DOI: 10.1016/j.bios.2015.12.013
Keywords
beta-cyclodextrin functionalized graphene nanosheet; Host-guest interaction; Pt/PdCu nanocube; Sandwich-type electrochemical immunosensor
Categories
Funding
- National Natural Science Foundation of China [21175057, 21375047, 21377046, 21575050, 21505051]
- Science and Technology Plan Project of Jinan [201307010]
- Science and Technology Development Plan of Shandong Province [2014GSF120004]
- Special Project for Independent Innovation and Achievements Transformation of Shandong Province [2014ZZCX05101]
- Graduate Innovation Foundation of University of Jinan (GIFUJN)
- Special Foundation for Taishan Scholar Professorship of Shandong Province [ts20130937]
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In this study, a novel and ultrasensitive sandwich-type electrochemical immunosensor was designed for the quantitative detection of squamous cell carcinoma antigen (SCCA) based on the beta-cyclodextrin functionalized graphene nanosheet (CD-GN) and the ternary hollow Pt/PdCu nanocube anchored on three-dimensional graphene framework (Pt/PdCu-3DGF). CD-GN exhibited high specific surface area and good dispersibility and stability in water, which were beneficial to fix captured antibodies (Ab(1)) through the supramolecular host-guest interaction between CD and Ab(1). The abundant oxygen-containing functional groups on 3DGF provided binding sites for anchoring noble metal nanoparticles. Pt/PdCu-3DGF could capture detected antibodies via the interaction of Pd-NH2 and Pt-NH2. Furthermore, the ternary metal nanoparticles exhibited high electrocatalytic activity toward the reduction of hydrogen peroxide. Under optimal conditions, the fabricated immunosensor showed a sensitive response to SCCA with two linear ranges. The linear ranges are 0.0001-1 ng/mL and 1-30 ng/mL with a detection limit of 25 fg/mL Additionally, the proposed immunosensor showed good reproducibility and stability. (C) 2015 Elsevier B.V. All rights reserved.
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