Journal
BIOSENSORS & BIOELECTRONICS
Volume 85, Issue -, Pages 198-204Publisher
ELSEVIER ADVANCED TECHNOLOGY
DOI: 10.1016/j.bios.2016.05.019
Keywords
Polydiacetylene; MicroRNA; Optical waveguide; Biosensing
Categories
Funding
- National Natural Science Foundation of China [51273186, 21574120, 91027024, 61427818, 11374286]
- National Key Basic Research Program of China [2013CBA01703, 2012CB921900, 2012CB922003]
- Chinese Academy of Sciences [kjcx2-yw-m11]
- Basic Research Fund for the Central Universities [WK2060200012]
- Science and Technological Fund of Anhui Province for Outstanding Youth [1608085J01, 1608085J02]
- National Institute of Health [GM107986, EB006521, EB018959, OD019975]
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Development of rapid, highly selective and sensitive miRNA detection in a complex biological environment has attracted considerable attention. Herein, we describe a novel two step method to construct gold-nanorod functionalized polydiacetylene (PDA) microtube for miRNA detection. In PDA microtube, with a one-dimensional (1D) waveguide nature, the excitation position and emission out-coupling position are far apart, thus helpful in reducing contribution of auto-fluorescence from biological sample. The use of specially designed toehold-mediated strand displacement reaction enables the reliable and selective discrimination of miRNA sequences with high sequence homology. Based on the condensing enrichment effect, the detection limit of the proposed PDA microtube system is as low as 0.01 nM, and it can be applied directly to detect disease-specific miRNA targets in human serum. This PDA microtube waveguide system can be further integrated into the chip for the potential applications in minimally invasive, portable clinical diagnostic equipment. (C) 2016 Elsevier B.V. All rights reserved.
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