Journal
BIOSENSORS & BIOELECTRONICS
Volume 79, Issue -, Pages 98-107Publisher
ELSEVIER ADVANCED TECHNOLOGY
DOI: 10.1016/j.bios.2015.12.005
Keywords
LFA; POC; Nucleic acid hybridization; Antigen-antibody interaction; Temperature; RH; Portable temperature-humidity control device
Categories
Funding
- Major International Joint Research Program of China [11120101002]
- International Science & Technology Cooperation Program of China [2013DFG02930]
- Ministry of Higher Education (MOHE), Government of Malaysia [UM.C/HIR/MOHE/ENG/44]
- China Young 1000-Talent Program
- Program for New Century Excellent Talents in University [NCET-12-0437]
Ask authors/readers for more resources
Lateral flow assays (LFAs) have currently attracted broad interest for point-of-care (POC) diagnostics, but their application has been restricted by poor quantification and limited sensitivity. While the former has been currently solved to some extent by the development of handheld or smartphone-based readers, the latter has not been addressed fully, particularly the potential influences of environmental conditions (e.g., temperature and relative humidity (RH)), which have not yet received serious attention. The present study reports the use of a portable temperature-humidity control device to provide an optimum environmental requirement for sensitivity improvement in LFAs, followed by quantification by using a smartphone. We found that a RH beyond 60% with temperatures of 55-60 degrees C and 37-40 degrees C produced optimum nucleic acid hybridization and antigen-antibody interaction in LFAs, respectively representing a 10-fold and 3-fold signal enhancement over ambient conditions (25 degrees C, 60% RH). We envision that in the future the portable device could be coupled with a fully integrated paper-based sample-to-answer biosensor for sensitive detection of various target analytes in POC settings. (C) 2015 Elsevier B.V. All rights reserved.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available