Journal
BIOSENSORS & BIOELECTRONICS
Volume 86, Issue -, Pages 849-857Publisher
ELSEVIER ADVANCED TECHNOLOGY
DOI: 10.1016/j.bios.2016.07.082
Keywords
TDG; Iridium(III) complex; G-quadruplex; Luminescence
Categories
Funding
- Hong Kong Baptist University [FRG2/14-15/004, FRG2/15-16/002]
- Health and Medical Research Fund [HMRF/14130522]
- Research Grants Council [HKBU/201811, HKBU/204612, HKBU/201913]
- French Agence Nationale de la Recherche/Research Grants Council Joint Research Scheme [AHKBU201/12]
- National Natural Science Foundation of China [21575121]
- Guangdong Province Natural Science Foundation [2015A030313816]
- Hong Kong Baptist University Century Club Sponsorship Scheme
- Interdisciplinary Research Matching Scheme [RC-IRMS/14-15/06]
- Science and Technology Development Fund, Macao SAR [098/2014/A2]
- University of Macau [MYRG091(Y3-L2)-ICMS12-LCH, MYRG2015-00137-ICMS-QRCM, MRG044/LCH/2015/ICMS]
- French Agence Nationale de la Recherche/Research Grants Council Joint Research Scheme (Oligoswitch) [ANR-12-IS07-0001]
Ask authors/readers for more resources
Thymine DNA glycosylase (TDG) performs essential functions in maintaining genetic integrity and epigenetic regulation, which also plays an essential role in DNA demethylation. In this work, the novel iridium(III) complex 1 with an anchor tail was synthesized and employed to construct a G-quadruplex-based assay for detecting TDG activity in aqueous solution by using the mismatched base excising property of TDG with T4 DNA ligase and phi29 DNA polymerase, in concert with the rolling circle amplification (RCA) strategy. The assay achieved a detection limit of 0.048 U mL(-1) (0.012 ng mL(-1)), and showed high selectivity towards TDG even in the presence of other proteins and enzymes. Additionally, the assay could function in diluted cellular debris. (C) 2016 Elsevier B.V. All rights reserved.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available
Share Paper
