Journal
BIOSENSORS & BIOELECTRONICS
Volume 85, Issue -, Pages 777-784Publisher
ELSEVIER ADVANCED TECHNOLOGY
DOI: 10.1016/j.bios.2016.05.090
Keywords
Platinum nanoparticle; Catalase-like activity; Microfluidic; Point-of-care test; Signal amplification
Categories
Funding
- Thousand Talents Program for Young Researchers
- National Natural Science Foundation of China [81160076]
- Fostering Talents Project for Young Academic and Technology leaders in Yunnan Province [2012HB029]
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Platinum nanoparticles incorporated volumetric bar-chart chip (PtNPs-V-Chip) is able to be used for point-of-care tests by providing quantitative and visualized readout without any assistance from instruments, data processing, or graphic plotting. To improve the sensitivity of PtNPs-V-Chip, hybridization chain reaction was employed in this quantitation platform for highly sensitive assays that can detect as low as 16 pM Ebola Virus DNA, 0.01 ng/mL carcinoembryonic antigen (CEA), and the 10 HER2-expressing cancer cells. Based on this amplified strategy, a 100-fold decrease of detection limit was achieved for DNA by improving the number of platinum nanoparticle catalyst for the captured analyte. This quantitation platform can also distinguish single base mismatch of DNA hybridization and observe the concentration threshold of CEA. The new strategy lays the foundation for this quantitation platform to be applied in forensic analysis, biothreat detection, clinical diagnostics and drug screening. (C) 2016 Elsevier B.V. All rights reserved.
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