Journal
BIOSENSORS & BIOELECTRONICS
Volume 79, Issue -, Pages 307-312Publisher
ELSEVIER ADVANCED TECHNOLOGY
DOI: 10.1016/j.bios.2015.12.051
Keywords
MicroRNA-21; Gastric cancer; Electrochemical biosensor; T7 exonuclease; T4 RNA ligase 2
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Funding
- National Natural Science Foundation of China [21105056, 21375079]
- Foundation of State Key Laboratory of Crop Biology [2014KF12]
- Natural Science Foundation of Shandong Province, China [ZR2014BQ029]
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MicroRNA (miRNA) is riveting nowadays due to its close relevance to human malignancies. Exploiting a fast and convenient biosensor for sensitive and specific detecting miRNA is necessary and it has significant meaning for oncology. In this work, to understand the relationship between miRNA-21 and gastric cancer, we employ T4 RNA ligase 2 to initiate specific ligation reaction depending on the sequence of target RNA, and T7 exonuclease to catalyze the first stage cyclic enzymatic amplification method (CEAM) specifically, which also resting on the target RNA sequence, and the second stage CEAM to further amplify the response resulting from the initial target RNA. Our two-stage CEAM strategy can detect miRNA-21 as low as 0.36 fM with remarkable specificity, and most importantly, it can be used to inspect the expression level of miRNA-21 in blood serum of gastric cancer patients. This will offer new perspective for figuring out the pathways of miRNA-21 involving in cancer. (C) 2015 Elsevier B.V. All rights reserved.
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