Journal
BIOSENSORS & BIOELECTRONICS
Volume 79, Issue -, Pages 758-762Publisher
ELSEVIER ADVANCED TECHNOLOGY
DOI: 10.1016/j.bios.2016.01.014
Keywords
Molecular beacon; Helper probe; Enzyme-free; Fluorescence; Amplification
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Funding
- Research Grants Council of the Hong Kong Special Administrative Region [16205714]
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We report an enzyme-free, sensitive strategy for DNA detections through fluorescence amplification. The sensing method employs molecular beacons (MBs) and two single-stranded helper DNA probes. In the presence of a DNA target, it binds and opens an MB. This triggers the hybridizations between the MB and helper probes, and consequently releases the DNA target, which becomes available to react with another MB and enhances the fluorescence emission of the MBs. The detection limit of the proposed strategy is 0.58 pM, which is about 3 orders of magnitude better than the conventional MB-based method. This method is also fast and exhibits good selectivity. It is superior to previous MB-based amplification approaches employing enzymes or nanomaterials. (C) 2016 Elsevier B.V. All rights reserved.
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