Sulfide-oxidizing bacteria community in full-scale bioscrubber treating H2S in biogas from swine anaerobic digester

The industrial-scale of hydrogen sulfide (H2S) removal system using biological process relies on the performance of sulfide-oxidizing bacteria (SOB) to reduce corrosive H2S before being used as fuel in an industrial boiler or electric generator. The sulfide-oxidizing bacteria community in the bioscrubber treating H2S in biogas from swine farm based on the 16S rRNA gene and Sox gene sequences analysis. Microbial sludge from SPM Feedmill Co., Ltd. (SPM swine farm) were collected from the inlet and outlet sampling ports of the bioscrubber. Sequencing of full-length 165 rRNA gene and next-generation sequencing (NGS) of short-read 16S region were employed to identify the SOB communities. The cultural dependent technique has been applied for isolation of pure SOB strains, including Acinetobacter towneri (MF765755), Enterobacter asburiae (MF765756) and Aeromonas veronii (MK659586). Together with NGS analysis, which showed bacteria belong to the genera Sulfurovum (37%), Sulfuricurvum (17%) and Thiothrix (9%) could play an important role in oxidized H2S in biogas. Therefore, these SOB genera: Acinetobacter, Aeromonas, Enterobacter, Sulfurovum, and Sulfuricurvum can be applied as an indicator for efficiency and stability of H2S treatment systems in biogas from swine farms. (C) 2019 Elsevier Ltd. All rights reserved.