Journal
REGENERATIVE MEDICINE
Volume 14, Issue 10, Pages 955-967Publisher
FUTURE MEDICINE LTD
DOI: 10.2217/rme-2019-0098
Keywords
Cutibacterium acnes; Propionibacterium acnes; anti-infective; antimicrobial; disc degeneration; intervertebral disc; leukocyte; low back pain; neutrophil; platelet-rich plasma; regenerative medicine
Categories
Funding
- Regenerative SportsCare Foundation
- EmCyte Corporation
Ask authors/readers for more resources
Aim: The most common risk associated with intradiscal injection of platelet-rich plasma (PRP) is discitis with Cutibacterium acnes. It is hypothesized that antimicrobial activity of PRP can be enhanced through inclusion of leukocytes or antibiotics in the injectate. Materials & methods: Multiple PRP preparations of varying platelet and leukocyte counts were co-cultured with C. acnes with or without cefazolin, with viable bacterial colony counts being recovered at 0, 4, 24 and 48 hours post-inoculation. Results: A direct correlation between C. acnes recovery and granulocyte counts were observed. Conclusion: We observed the greatest antimicrobial activity with the leukocyte-rich, high platelet PRP preparation combined with an antibiotic in the injectate. However, cefazolin did not completely clear the bacteria in this assay.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available