Journal
BIOSCIENCE BIOTECHNOLOGY AND BIOCHEMISTRY
Volume 80, Issue 8, Pages 1546-1554Publisher
OXFORD UNIV PRESS
DOI: 10.1080/09168451.2016.1179094
Keywords
aequorin; auxin; calcium; Oryza sativa L; ROS
Categories
Funding
- Regional Innovation Cluster Program
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Indole-3-acetic acid (IAA) is the major natural auxin involved in the regulation of a variety of growth and developmental processes such as division, elongation, and polarity determination in growing plant cells. It has been shown that dividing and/or elongating plant cells accompanies the generation of reactive oxygen species (ROS) and a number of reports have suggested that hormonal actions can be mediated by ROS through ROS-mediated opening of ion channels. Here, we surveyed the link between the action of IAA, oxidative burst, and calcium channel activation in a transgenic cells of rice expressing aequorin in the cytosol. Application of IAA to the cells induced a rapid and transient generation of superoxide which was followed by a transient increase in cytosolic Ca2+ concentration ([Ca2+](c)). The IAA-induced [Ca2+](c) elevation was inhibited by Ca2+ channel blockers and a Ca2+ chelator. Furthermore, ROS scavengers effectively blocked the action of IAA on [Ca2+](c) elevation.
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