Journal
BIOSCIENCE BIOTECHNOLOGY AND BIOCHEMISTRY
Volume 80, Issue 4, Pages 706-711Publisher
TAYLOR & FRANCIS LTD
DOI: 10.1080/09168451.2015.1123604
Keywords
miR-143-3p; C2C12; myoblast differentiation; Wnt pathway
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Funding
- Sichuan Sci & Tech Support Program [2013NZ0041]
- earmarked fund for China Agriculture Research System [CARS-36-05B]
- Chinese National Sci & Tech Support Program [2013BAD20B07]
- Program for Changjiang Scholars and Innovative Research Team in University [IRT13083]
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MicroRNAs are a class of 18-22 nucleotide non-coding RNAs that modulate gene expression by associating with the 3 untranslated regions of mRNAs. A large number of microRNAs are involved in the regulation of myoblast differentiation, many of which remain undiscovered. In this study, we found that miR-143-3p was upregulated during C2C12 myoblast differentiation and over-expression of miR-143-3p significantly inhibited the relative expression levels of MyoD, MyoG, myf5, and MyHC genes, especially in the later stages of differentiation. In addition, miR-143-3p inhibited expression of genes involved in the endogenous Wnt signaling pathway during C2C12 myoblast differentiation, including Wnt5a, LRP5, Axin2, and -catenin. These results indicate that miR-143-3p represents a new myogenic differentiation-associated microRNA that can inhibit C2C12 myoblast differentiation, especially in the later stages of differentiation.
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