Journal
BIOSCIENCE BIOTECHNOLOGY AND BIOCHEMISTRY
Volume 80, Issue 8, Pages 1470-1477Publisher
TAYLOR & FRANCIS LTD
DOI: 10.1080/09168451.2016.1165600
Keywords
hepatic stellate cell; tenascin-C(TN-C); type I collagen(Col I); alpha 9 beta 1 integrin; TGF-beta 1
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Funding
- Shaanxi Province Science and Technology Plan Project of Social Development Plans for Public Relations [S2009SF471, 2012SF2-13, 2015SF068]
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Tenascin-C (TN-C) is an extracellular matrix glycoprotein markedly upregulated during liver fibrosis. The study is performed to explore the role of TN-C during the growth and activation of hepatic stellate cells (HSCs). We found that TN-C was accumulated accompanying with the HSC activation. Our data on cell migration assay revealed that the rTN-C treatment enhanced HSC migration in a dose- and time-dependent manner, but did not influence their proliferation. HSCs transfected with pTARGET-TN-C overexpression vector displayed increased the type I collagen (Col I) production. TN-C overexpression enhanced the process of HSC activation through TGF-1 signaling. Moreover, the anti-91 integrin antibody treatment blocked the TN-C-driven Col I increase in rat HSCs. Collectively, TN-C had a positive role in activation of HSCs mediated by TGF-1 and 91 integrin, manifesting elevation of Col I production and promotion of cell migration. Our results provide a potential insight for the therapy of hepatic fibrosis.
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