Journal
PROCESS BIOCHEMISTRY
Volume 90, Issue -, Pages 50-57Publisher
ELSEVIER SCI LTD
DOI: 10.1016/j.procbio.2019.11.017
Keywords
Bacillus amyloliquefaciens; Iturin A; Promoter replacement; AbrB; Medium optimization
Categories
Funding
- National Key Research and Development Program of China [2018YFA090039]
- Technical Innovation Special Fund of Hubei Province [2018ACA149]
- Science and Technology Project of Hubei Tobacco Company [027Y2019-018]
- Key Technology Project of China National Tobacco Corporation [110201502014, 110201502018]
Ask authors/readers for more resources
Brown spot disease, caused by Alternaria alternate, is one of the most destructive leaf spot diseases that made a major impact on tobacco growing. Biocontrol has attracted increasing attentions as its features of environmental friendship, promoting plant growth, etc. Iturin A, with broad spectrum antifungal activity, is a kind of biosurfactant that mainly produced by Bacillus. In this study, the promoter of iturin A synthetase cluster of Bacillus amyloliquefaciens HZ-12 was respectively replaced by promoters P43, PbacA, PsrfA and Pylb, our results implied that transcriptional level of gene ituD and iturin A titer showed consistent change trends, and PbacA was proven as the most efficient promoter, iturin A titer of which reached 950.08 +/- 19.43 mg/L. Furthermore, regulator gene abrB was deleted to release the repression effect of AbrB on PbacA, ituD transcriptional level and iturin A titer were increased by 133.25 % and 20.88 %, respectively. Then, the maximum iturin A titer reached 2013.43 +/- 32.86 mg/L by optimizing fermentation medium, increased by 392.15 % compared to the original (408.97 +/- 21.35 mg/L). Finally, our results demonstrated that enhancing iturin A synthetic capability benefited the suppression of A. alternate. Collectively, this study provided a promising strain with an efficient fermentation medium for large-scale industrial production of iturin A.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available