Journal
PROCESS BIOCHEMISTRY
Volume 89, Issue -, Pages 98-109Publisher
ELSEVIER SCI LTD
DOI: 10.1016/j.procbio.2019.09.036
Keywords
Pine bark extract; Gold nanoparticles; Human serum albumin; HEK293 cells; Oleamide
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Funding
- University of KwaZulu-Natal (UKZN)
- Department of Science and Technology, New Delhi under PURSE 2nd phase scheme [SR/PURSE 2/38 (G)]
- Department of Science and Technology, New Delhi under MHRD-RUSA 2.0 scheme [F.24-51/2014-U Policy]
- UGC, New Delhi [F. 14-13/2013 (Inno/ASIST)]
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Unique characteristics displayed by phytoconstituent conjugated nanoparticles and their crucial interactions with proteins serve to develop nanoparticle-bio-interface platform. Gold nanoparticles of 16 nm in size were generated using aqueous extracts of pine bark and further conjugated to human serum albumin. The gold nanoparticles-protein complex was characterized by surface plasmon resonance, UV-vis and emission spectroscopy techniques. Further, it was characterized for surface morphology and elemental composition, crystallographic quality, nanoparticles size, shape, stability, structural determination and the identification of capping agent. Moreover, the interaction of gold nanoparticles with human serum albumin was investigated using conventional spectroscopy techniques. Fluorescence quenching and absorption studies demonstrated an effective binding of human serum albumin with oleamide capped gold nanoparticles. The molecular docking study showed a binding affinity of -6.1 kcal/mol whereas the molecular dynamics simulation indicated that the binding of oleamide to human serum albumin. A biological evaluation of pine bark extract-gold nanoparticles showed cytotoxicity with increased cell mortality in lung cancer cells and minimal toxicity on non-cancerous human embryonic kidney cells, respectively.
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